Netherlands VWO 6 (eindexamenjaar) Biology
Chapters: 4
1. Self-regulation (part 3)
Protein synthesis
- Molecular Basis of Inheritance – DNA is the genetic material in most living things (some viruses use RNA). It is a double helix: two antiparallel strands of nucleotides, A pairs with T by 2 hydrogen bonds and G with C by 3. Long DNA is packed on histones into nucleosomes and then chromatin. DNA copies itself semi-conservatively (Meselson–Stahl). The central dogma says DNA → RNA → protein. Transcription makes RNA from one strand; in eukaryotes the hnRNA is capped, tailed and spliced. The genetic code is a triplet, has 64 codons (61 for amino acids, 3 stops), starts with AUG, is nearly universal and degenerate. Ribosomes translate mRNA into protein with tRNA adaptors. Genes are switched on and off; the lac operon is the classic example. The Human and Rice Genome Projects read whole genomes, and DNA fingerprinting uses repeat DNA (VNTRs) to identify people.
2. Self-organisation (part 2)
Self-organisation of cells · Self-organisation of ecosystems
- Molecular Basis of Inheritance – DNA is the genetic material in most living things (some viruses use RNA). It is a double helix: two antiparallel strands of nucleotides, A pairs with T by 2 hydrogen bonds and G with C by 3. Long DNA is packed on histones into nucleosomes and then chromatin. DNA copies itself semi-conservatively (Meselson–Stahl). The central dogma says DNA → RNA → protein. Transcription makes RNA from one strand; in eukaryotes the hnRNA is capped, tailed and spliced. The genetic code is a triplet, has 64 codons (61 for amino acids, 3 stops), starts with AUG, is nearly universal and degenerate. Ribosomes translate mRNA into protein with tRNA adaptors. Genes are switched on and off; the lac operon is the classic example. The Human and Rice Genome Projects read whole genomes, and DNA fingerprinting uses repeat DNA (VNTRs) to identify people.
3. Interaction (part 2)
Molecular and cellular interaction · Interaction in ecosystems
- Gene Regulation: How Cells Switch Genes On and Off – Every cell carries the same DNA but uses only some genes. Cells control mostly at transcription. In bacteria, the lac operon is a set of genes under one promoter and one operator: a repressor blocks it when there is no lactose; lactose removes the repressor and the genes are read (an inducible operon). In eukaryotes, transcription factors, enhancers and silencers control each gene, and epigenetic marks (DNA methylation, histone packing) can keep genes off without changing the DNA code. Different on/off patterns make different cell types (differentiation).
4. Evolution
Selection · Speciation · Biodiversity · Origin of life
- Evolution: Origin of Life, Mechanisms and Human Evolution – Life began on the early Earth from simple chemicals: Oparin and Haldane proposed it and Miller made amino acids in a flask. Evidence of evolution comes from fossils, homologous and analogous organs, embryos, molecules and changes we can watch (industrial melanism, drug resistance). Darwin explained it by natural selection acting on variation in populations; the modern synthetic theory adds genes: mutation, recombination, gene flow, genetic drift and natural selection change allele frequencies. If none of these act, frequencies stay constant: Hardy–Weinberg, p² + 2pq + q² = 1. Selection can be stabilising, directional or disruptive. One ancestor spreading into many habitats gives adaptive radiation (Darwin’s finches, Australian marsupials). Humans evolved from Dryopithecus-like apes through Australopithecus, Homo habilis, Homo erectus and Neanderthals to Homo sapiens.
- Population Genetics: Gene Pools, Hardy–Weinberg and New Species – Population genetics studies how allele frequencies change in a population. The gene pool is all alleles of all individuals. For one gene with alleles A and a, p + q = 1. If a population is large, mates randomly, and has no migration, mutation or selection, the Hardy–Weinberg principle says frequencies stay the same and genotypes are p² (AA) + 2pq (Aa) + q² (aa) = 1. Any change from this means evolution is happening, caused by genetic drift (bottleneck, founder effect), gene flow, mutation, natural selection or non-random mating. A species, in the biological species concept, is a group whose members can interbreed: a shared gene pool. New species form when gene pools are cut off by reproductive isolation: allopatric (geographic barrier) or sympatric (same area, e.g. polyploidy, habitat or behaviour). One ancestor can split into many forms (adaptive radiation, divergence), while unrelated groups can evolve similar shapes (convergence).
- Biodiversity and its Conservation – Biodiversity is the variety of life at three levels: genetic, species and ecological. It is highest near the equator and grows with area (log S = log C + Z log A). Every species matters, like rivets on a plane. We are losing species fast because of the 'evil quartet': habitat loss and fragmentation, over-exploitation, alien species invasions and co-extinction. The IUCN Red List (Red Data Book) ranks species from Least Concern to Extinct. We protect life in situ (hotspots, national parks, sanctuaries, biosphere reserves, sacred groves, Ramsar wetlands) and ex situ (zoos, botanical gardens, seed and gene banks).