National Year 13 Biology
Chapters: 4
1. 3.5 Energy transfers in and between organisms
3.5.1 Photosynthesis · 3.5.2 Respiration · 3.5.3 Energy and ecosystems · 3.5.4 Nutrient cycles
- Photosynthesis in Higher Plants – Green plants make sugar from carbon dioxide and water using light. It happens in chloroplasts. The light reaction in the thylakoids splits water, gives out oxygen and makes ATP and NADPH. The Calvin cycle in the stroma uses that ATP and NADPH to fix CO₂ into sugar. C₄ plants add a CO₂ pump that stops the wasteful photorespiration. The slowest factor, such as light, CO₂ or temperature, sets the rate.
- Respiration in Plants – Respiration breaks food like glucose to release energy stored as ATP. Plants take in O₂ through stomata and lenticels. Glycolysis in the cytoplasm splits glucose into two pyruvates. Without O₂, pyruvate is fermented to ethanol or lactic acid. With O₂, pyruvate enters the mitochondrion, runs the Krebs cycle, and the electron transport system uses O₂ to make most of the ATP: up to 38 per glucose. The same path also builds molecules (amphibolic). RQ = CO₂ given out ÷ O₂ taken in.
- Ecosystem: Structure, Productivity, Energy Flow and Pyramids – An ecosystem is a working unit of nature where living things (biotic) and non-living things (abiotic) interact. Plants make food at a rate called productivity: gross (GPP) minus respiration gives net (NPP = GPP − R). Dead matter is broken down in five steps: fragmentation, leaching, catabolism, humification and mineralisation. Energy enters as sunlight, flows one way through trophic levels and only about 10% passes on each time. Ecological pyramids of number, biomass and energy show this; number and biomass pyramids can be inverted, but the energy pyramid is always upright.
- Nutrient Cycles: How Nitrogen and Phosphorus Are Reused – Living things need nitrogen (for amino acids, proteins, DNA and ATP) and phosphorus (for DNA, ATP and phospholipids). The amount on Earth is fixed, so these elements are recycled. Saprobionts (decomposer fungi and bacteria) digest dead matter outside their cells and release mineral ions. In the nitrogen cycle, nitrogen fixation turns N₂ gas into ammonium, ammonification releases ammonium from dead matter, nitrification turns ammonium into nitrite and then nitrate (needs oxygen), and denitrification turns nitrate back into N₂ (no oxygen). The phosphorus cycle has no gas stage: phosphate comes from weathered rock, passes through food chains and returns through decomposers, then forms sediment and new rock over very long times. Mycorrhizal fungi act like extra roots and help plants take up water and phosphate. Fertilisers replace ions lost when crops are harvested, but extra fertiliser can be washed out of soil (leaching) and cause eutrophication, which kills fish by removing oxygen from water.
2. 3.6 Organisms respond to changes in their internal and external environments
3.6.1 Stimuli and responses · 3.6.2 Nervous coordination · 3.6.3 Skeletal muscle contraction · 3.6.4 Homeostasis
- Stimulus and Response – A stimulus is a change in the surroundings or inside the body. Living things that detect it and respond have a better chance of surviving. The path is always stimulus → receptor → coordinator → effector → response. Simple organisms move by taxis (towards or away) or kinesis (speed changes). Plants bend by tropisms using the growth factor IAA. Animals use receptors such as the Pacinian corpuscle and the rods and cones of the eye, reflex arcs for fast protection, and the brain's medulla to change the heart rate.
- Neural Control and Coordination – Coordination means organs working together. The nervous system does this fast, using neurons. A neuron has a cell body, dendrites and an axon; many axons have a myelin sheath with nodes of Ranvier. The central nervous system (brain and spinal cord) processes information; the peripheral nervous system (nerves) carries it. The PNS has a somatic part (skeletal muscles) and an autonomic part (sympathetic and parasympathetic) for internal organs, the visceral system. At rest the axon is polarised: inside −, outside +, about −70 mV. A stimulus lets Na+ rush in, creating an action potential that travels along the axon. At a synapse, neurotransmitters carry the signal to the next neuron.
- Locomotion and Movement – Movement is a change in position of a body part; locomotion is moving the whole body from place to place. Cells move in three ways: amoeboid, ciliary and flagellar. Humans use muscular movement. Muscles are skeletal, visceral or cardiac. A skeletal muscle is made of fibres, each packed with myofibrils made of repeating sarcomeres of actin and myosin. A nerve signal releases Ca2+, myosin heads pull actin using ATP, and the sarcomere shortens (sliding filament theory). The skeleton has 206 bones. Joints can be fibrous, cartilaginous or synovial (ball and socket, hinge, pivot, gliding, saddle). Disorders include myasthenia gravis, tetany, muscular dystrophy, arthritis, osteoporosis and gout.
- Homeostasis – Homeostasis is how the body keeps its internal environment (blood and tissue fluid) steady even when the outside world changes. It controls body temperature (about 37 °C), blood glucose (about 4–6 mmol/L), water and salt, and blood pH (about 7.4). It mostly uses negative feedback: a receptor senses a change, a control centre decides, and an effector brings the level back to its set point.
3. 3.7 Genetics, populations, evolution and ecosystems
3.7.1 Inheritance · 3.7.2 Populations · 3.7.3 Evolution and speciation · 3.7.4 Populations in ecosystems
- Principles of Inheritance and Variation – Genes come in pairs (alleles). Each parent passes one allele of each pair to the child. Mendel showed dominance (3 : 1), segregation and independent assortment (9 : 3 : 3 : 1). Real life has twists: incomplete dominance (1 : 2 : 1), co-dominance (AB blood), multiple alleles, pleiotropy (one gene, many effects) and polygenic traits (many genes, one trait). Genes sit on chromosomes, so genes on the same chromosome are linked and are separated only by crossing over. Sex chromosomes decide sex (XX-XY in humans, ZW in birds, haplo-diploid in honeybees) and carry sex-linked genes (haemophilia, colour blindness). Mistakes in genes cause Mendelian disorders (thalassemia, sickle-cell) and mistakes in chromosome number cause Down, Turner and Klinefelter syndromes.
- Evolution: Origin of Life, Mechanisms and Human Evolution – Life began on the early Earth from simple chemicals: Oparin and Haldane proposed it and Miller made amino acids in a flask. Evidence of evolution comes from fossils, homologous and analogous organs, embryos, molecules and changes we can watch (industrial melanism, drug resistance). Darwin explained it by natural selection acting on variation in populations; the modern synthetic theory adds genes: mutation, recombination, gene flow, genetic drift and natural selection change allele frequencies. If none of these act, frequencies stay constant: Hardy–Weinberg, p² + 2pq + q² = 1. Selection can be stabilising, directional or disruptive. One ancestor spreading into many habitats gives adaptive radiation (Darwin’s finches, Australian marsupials). Humans evolved from Dryopithecus-like apes through Australopithecus, Homo habilis, Homo erectus and Neanderthals to Homo sapiens.
- Organisms and Populations: Growth and Interactions – A population is a group of the same species living in one area at one time. It has features that a single organism does not have: density, birth rate, death rate, sex ratio and age structure. N grows by births and immigration and shrinks by deaths and emigration. With unlimited food it grows fast (J-curve, dN/dt = rN); with limited food it slows and stops at the carrying capacity K (S-curve, dN/dt = rN(1 − N/K)). Different species affect each other: mutualism (+/+), competition (−/−), predation and parasitism (+/−), commensalism (+/0) and amensalism (−/0).
4. 3.8 The control of gene expression
3.8.1 Gene mutations and protein structure · 3.8.2 Control of gene expression · 3.8.3 Using genome projects · 3.8.4 Gene technologies
- Mutations – A mutation is a sudden, lasting change in the DNA. Gene (point) mutations change one or a few bases: substitution, insertion or deletion. Insertions and deletions shift the reading frame. Chromosome mutations change big pieces or the number of chromosomes. Mutations happen by chance or because of mutagens such as UV light, X-rays and some chemicals. They can be harmful, neutral or useful, and they are the first source of all genetic variation.
- Molecular Basis of Inheritance – DNA is the genetic material in most living things (some viruses use RNA). It is a double helix: two antiparallel strands of nucleotides, A pairs with T by 2 hydrogen bonds and G with C by 3. Long DNA is packed on histones into nucleosomes and then chromatin. DNA copies itself semi-conservatively (Meselson–Stahl). The central dogma says DNA → RNA → protein. Transcription makes RNA from one strand; in eukaryotes the hnRNA is capped, tailed and spliced. The genetic code is a triplet, has 64 codons (61 for amino acids, 3 stops), starts with AUG, is nearly universal and degenerate. Ribosomes translate mRNA into protein with tRNA adaptors. Genes are switched on and off; the lac operon is the classic example. The Human and Rice Genome Projects read whole genomes, and DNA fingerprinting uses repeat DNA (VNTRs) to identify people.
- Biotechnology: Principles and Processes (Recombinant DNA Technology) – Genetic engineering means changing the genes of a living thing on purpose. We cut the gene we want with restriction enzymes (molecular scissors), paste it into a carrier DNA called a vector using DNA ligase (molecular glue), put it into a host cell, pick out the cells that took it, and grow them in big tanks (bioreactors) to collect the product. PCR makes millions of copies of a gene, and gel electrophoresis sorts DNA pieces by size.