Japan 高校2年 Biology (part 1)

Chapters: 3

1. Evolution

Origin of life and evolution of cells · Genetic change and mechanisms of evolution · Phylogeny and evolution

Evolution: Origin of Life, Mechanisms and Human Evolution – Life began on the early Earth from simple chemicals: Oparin and Haldane proposed it and Miller made amino acids in a flask. Evidence of evolution comes from fossils, homologous and analogous organs, embryos, molecules and changes we can watch (industrial melanism, drug resistance). Darwin explained it by natural selection acting on variation in populations; the modern synthetic theory adds genes: mutation, recombination, gene flow, genetic drift and natural selection change allele frequencies. If none of these act, frequencies stay constant: Hardy–Weinberg, p² + 2pq + q² = 1. Selection can be stabilising, directional or disruptive. One ancestor spreading into many habitats gives adaptive radiation (Darwin’s finches, Australian marsupials). Humans evolved from Dryopithecus-like apes through Australopithecus, Homo habilis, Homo erectus and Neanderthals to Homo sapiens.
Biological Classification – Whittaker (1969) sorted living things into five kingdoms, Monera, Protista, Fungi, Plantae and Animalia, using cell type, cell wall, body plan, mode of nutrition, reproduction and relationships. Monera are prokaryotes (bacteria, archaebacteria, cyanobacteria, mycoplasma). Protista are one-celled eukaryotes in five groups. Fungi are heterotrophs with chitin walls in four classes. Lichens are an alga and a fungus living together. Viruses, viroids and prions are not placed in any kingdom.

Cells and molecules · Metabolism

Cell: The Unit of Life – Every living thing is made of cells, and every new cell comes from an old cell. Prokaryotic cells (bacteria) have no nuclear envelope and no membrane-bound organelles; eukaryotic cells have a true nucleus and many organelles. The membrane is a fluid mosaic of lipids and proteins; plants add a cellulose wall. ER, Golgi, lysosomes and vacuoles form the endomembrane system. Mitochondria and plastids make energy and food, ribosomes make proteins, the cytoskeleton, cilia, flagella and centrioles give shape and movement, and the nucleus holds the DNA.
Respiration: Aerobic, Anaerobic and the Human Lungs – Respiration is the breakdown of food, usually glucose, inside cells to release energy, stored as ATP. With oxygen (aerobic) glucose is fully broken into carbon dioxide and water and gives much energy; without oxygen (anaerobic) it gives little energy and makes ethanol (yeast) or lactic acid (muscles). Our lungs bring oxygen to the blood through millions of alveoli.

Genetic information and expression · Development and gene expression · Gene technology

Molecular Basis of Inheritance – DNA is the genetic material in most living things (some viruses use RNA). It is a double helix: two antiparallel strands of nucleotides, A pairs with T by 2 hydrogen bonds and G with C by 3. Long DNA is packed on histones into nucleosomes and then chromatin. DNA copies itself semi-conservatively (Meselson–Stahl). The central dogma says DNA → RNA → protein. Transcription makes RNA from one strand; in eukaryotes the hnRNA is capped, tailed and spliced. The genetic code is a triplet, has 64 codons (61 for amino acids, 3 stops), starts with AUG, is nearly universal and degenerate. Ribosomes translate mRNA into protein with tRNA adaptors. Genes are switched on and off; the lac operon is the classic example. The Human and Rice Genome Projects read whole genomes, and DNA fingerprinting uses repeat DNA (VNTRs) to identify people.
Biotechnology: Principles and Processes (Recombinant DNA Technology) – Genetic engineering means changing the genes of a living thing on purpose. We cut the gene we want with restriction enzymes (molecular scissors), paste it into a carrier DNA called a vector using DNA ligase (molecular glue), put it into a host cell, pick out the cells that took it, and grow them in big tanks (bioreactors) to collect the product. PCR makes millions of copies of a gene, and gel electrophoresis sorts DNA pieces by size.